The influence of hypoxia on GATA-1 and Epo expression levels in developing zebrafish

Nonfiction, Science & Nature, Science, Biological Sciences, Zoology
Cover of the book The influence of hypoxia on GATA-1 and Epo expression levels in developing zebrafish by Markus Holotta, GRIN Publishing
View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart
Author: Markus Holotta ISBN: 9783638900270
Publisher: GRIN Publishing Publication: January 25, 2008
Imprint: GRIN Publishing Language: English
Author: Markus Holotta
ISBN: 9783638900270
Publisher: GRIN Publishing
Publication: January 25, 2008
Imprint: GRIN Publishing
Language: English

Master's Thesis from the year 2007 in the subject Biology - Zoology, grade: 1,0, University of Innsbruck (Zoologie), 90 entries in the bibliography, language: English, abstract: The transcription factor GATA-1 is essential for the development of the erythroid cell lineage in vertebrates. In this article we introduce a method to easily determine the approximately development status of red blood cells and the progression of blood formation by intensity of fluorescence in GATA-1/Ds-Red marked zebrafish. We classified the blood cells on the basis of their fluorescence intensity in 5 intensity stages (IS) with the brightest in IS 1. The comparison with our erythropoietin (Epo) data showed a noticable correlation between GATA-1, Epo mRNA and EPO protein level. Between 2 and 3 dpf we observed a major increase in blood cell concentration to circa 1200 cells*nl-1, until 15 dpf this value decreased to about the half. The appearance of IS 1 cells correspond approximately to the peaks in Epo cRNA copies and the highest values in EPO protein emerged about 1 day later. Our data show that the blood cell concentration, Epo and Gata-1 expression in zebrafish larvae is subjected to large fluctuations in the first few days of development.

View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart

Master's Thesis from the year 2007 in the subject Biology - Zoology, grade: 1,0, University of Innsbruck (Zoologie), 90 entries in the bibliography, language: English, abstract: The transcription factor GATA-1 is essential for the development of the erythroid cell lineage in vertebrates. In this article we introduce a method to easily determine the approximately development status of red blood cells and the progression of blood formation by intensity of fluorescence in GATA-1/Ds-Red marked zebrafish. We classified the blood cells on the basis of their fluorescence intensity in 5 intensity stages (IS) with the brightest in IS 1. The comparison with our erythropoietin (Epo) data showed a noticable correlation between GATA-1, Epo mRNA and EPO protein level. Between 2 and 3 dpf we observed a major increase in blood cell concentration to circa 1200 cells*nl-1, until 15 dpf this value decreased to about the half. The appearance of IS 1 cells correspond approximately to the peaks in Epo cRNA copies and the highest values in EPO protein emerged about 1 day later. Our data show that the blood cell concentration, Epo and Gata-1 expression in zebrafish larvae is subjected to large fluctuations in the first few days of development.

More books from GRIN Publishing

Cover of the book Leadership & Change - How to implement change in an all-male & all-scientist team? by Markus Holotta
Cover of the book The Placement of Clitics in Serbo-Croatian by Markus Holotta
Cover of the book Compounding - the general structure of compounds and their most striking features by Markus Holotta
Cover of the book Introducing the Dandy to the New World - Oscar Wilde visits America, January 2nd 1882 - December 27th 1882 by Markus Holotta
Cover of the book Folk Etymology as a Linguistic Phenomenon by Markus Holotta
Cover of the book 'Bare passives' and 'relative clauses' in be-passive form as modifiers by Markus Holotta
Cover of the book The Economic Model of Brazil during the Military Dictatorship by Markus Holotta
Cover of the book Immigration and the policy of language by Markus Holotta
Cover of the book Video On Demand - Television For A New Millenium by Markus Holotta
Cover of the book Gute Aufgaben im Mathematikunterricht der Grundschule by Markus Holotta
Cover of the book Hizb ut-Tahrir in Central Asia - messengers of a coming revolution? by Markus Holotta
Cover of the book Poverty in Brazil - What role do racial differences play? by Markus Holotta
Cover of the book Die Funktionen eines Galeristen als Intermediär by Markus Holotta
Cover of the book Pulp Fiction - An Analysis of Storyline and Characters by Markus Holotta
Cover of the book The Concepts of Honour and Revenge in Beowulf and Hamlet by Markus Holotta
We use our own "cookies" and third party cookies to improve services and to see statistical information. By using this website, you agree to our Privacy Policy