The influence of hypoxia on GATA-1 and Epo expression levels in developing zebrafish

Nonfiction, Science & Nature, Science, Biological Sciences, Zoology
Cover of the book The influence of hypoxia on GATA-1 and Epo expression levels in developing zebrafish by Markus Holotta, GRIN Publishing
View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart
Author: Markus Holotta ISBN: 9783638900270
Publisher: GRIN Publishing Publication: January 25, 2008
Imprint: GRIN Publishing Language: English
Author: Markus Holotta
ISBN: 9783638900270
Publisher: GRIN Publishing
Publication: January 25, 2008
Imprint: GRIN Publishing
Language: English

Master's Thesis from the year 2007 in the subject Biology - Zoology, grade: 1,0, University of Innsbruck (Zoologie), 90 entries in the bibliography, language: English, abstract: The transcription factor GATA-1 is essential for the development of the erythroid cell lineage in vertebrates. In this article we introduce a method to easily determine the approximately development status of red blood cells and the progression of blood formation by intensity of fluorescence in GATA-1/Ds-Red marked zebrafish. We classified the blood cells on the basis of their fluorescence intensity in 5 intensity stages (IS) with the brightest in IS 1. The comparison with our erythropoietin (Epo) data showed a noticable correlation between GATA-1, Epo mRNA and EPO protein level. Between 2 and 3 dpf we observed a major increase in blood cell concentration to circa 1200 cells*nl-1, until 15 dpf this value decreased to about the half. The appearance of IS 1 cells correspond approximately to the peaks in Epo cRNA copies and the highest values in EPO protein emerged about 1 day later. Our data show that the blood cell concentration, Epo and Gata-1 expression in zebrafish larvae is subjected to large fluctuations in the first few days of development.

View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart

Master's Thesis from the year 2007 in the subject Biology - Zoology, grade: 1,0, University of Innsbruck (Zoologie), 90 entries in the bibliography, language: English, abstract: The transcription factor GATA-1 is essential for the development of the erythroid cell lineage in vertebrates. In this article we introduce a method to easily determine the approximately development status of red blood cells and the progression of blood formation by intensity of fluorescence in GATA-1/Ds-Red marked zebrafish. We classified the blood cells on the basis of their fluorescence intensity in 5 intensity stages (IS) with the brightest in IS 1. The comparison with our erythropoietin (Epo) data showed a noticable correlation between GATA-1, Epo mRNA and EPO protein level. Between 2 and 3 dpf we observed a major increase in blood cell concentration to circa 1200 cells*nl-1, until 15 dpf this value decreased to about the half. The appearance of IS 1 cells correspond approximately to the peaks in Epo cRNA copies and the highest values in EPO protein emerged about 1 day later. Our data show that the blood cell concentration, Epo and Gata-1 expression in zebrafish larvae is subjected to large fluctuations in the first few days of development.

More books from GRIN Publishing

Cover of the book The linguistic situation in Zimbabwe by Markus Holotta
Cover of the book E-Health Services in Rural Communities of developing Countries by Markus Holotta
Cover of the book Examination of the Sea Resort Prora by Markus Holotta
Cover of the book The Metamorphosis of Alex in Stanley Kubrick's 'Clockwork Orange' from a Viewpoint of Abnormal Psychology by Markus Holotta
Cover of the book The Power of Motivational Events by Markus Holotta
Cover of the book The European social dialogue on sectoral level: transport by Markus Holotta
Cover of the book 'Remapping the Jungle...'? - 'Enlightening', 'white'-washing 'shadows' of Kant et al.!? by Markus Holotta
Cover of the book Partnership of convenience by Markus Holotta
Cover of the book Positioning and negation of identities in a bilingual intimate communication by Markus Holotta
Cover of the book American Deathways: The Meaning of Death in the American Indian Society by Markus Holotta
Cover of the book The Carnivalesque Flows: Art and Advertising by Markus Holotta
Cover of the book Selling Intervention by Markus Holotta
Cover of the book Phonological Characteristics of American English by Markus Holotta
Cover of the book The Credit on Real Estate - Local Value and Relevance for the Construction Financing in Germany and Denmark by Markus Holotta
Cover of the book The house as Gothic element in Anglo-American fiction (18th - 20th century) by Markus Holotta
We use our own "cookies" and third party cookies to improve services and to see statistical information. By using this website, you agree to our Privacy Policy