Human Cancer in Primary Culture, A Handbook

Nonfiction, Health & Well Being, Medical, Specialties, Oncology, Science & Nature, Science, Biological Sciences, Zoology
Cover of the book Human Cancer in Primary Culture, A Handbook by , Springer Netherlands
View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart
Author: ISBN: 9789401133043
Publisher: Springer Netherlands Publication: December 6, 2012
Imprint: Springer Language: English
Author:
ISBN: 9789401133043
Publisher: Springer Netherlands
Publication: December 6, 2012
Imprint: Springer
Language: English

Growing human cancer cells in primary culture requires patience, intuition, care and experience. This is one of the few areas where the wrinkled senior scientist can be more productive than the bright young post-doc. There are few mechanical aids, no automated procedures, and kits are unheard-of. There is no right way to do it and every tumour is different. But this book will make it easier! Chapter 1 on characterization is essential reading. Much published work is useless because of the failure to take two simple steps to characterise the cells. The first step is to fully record all the clinical data - the absence of this information can render the work valueless. The second step is to confirm the origin of the tissue to exclude cross­ contamination. The wastage of years of work can be avoided with the use of a simple DNA preparation with a couple of commercially-available probes. Chapter 2 describes the development of serum-free media. This is a goal many would like to achieve, particularly if someone else does it, as it is laborious and empirical. Defined serum-free medium is essential for studies of growth factors and has major advantages in the commercial preparation of cell products and other applications.

View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart

Growing human cancer cells in primary culture requires patience, intuition, care and experience. This is one of the few areas where the wrinkled senior scientist can be more productive than the bright young post-doc. There are few mechanical aids, no automated procedures, and kits are unheard-of. There is no right way to do it and every tumour is different. But this book will make it easier! Chapter 1 on characterization is essential reading. Much published work is useless because of the failure to take two simple steps to characterise the cells. The first step is to fully record all the clinical data - the absence of this information can render the work valueless. The second step is to confirm the origin of the tissue to exclude cross­ contamination. The wastage of years of work can be avoided with the use of a simple DNA preparation with a couple of commercially-available probes. Chapter 2 describes the development of serum-free media. This is a goal many would like to achieve, particularly if someone else does it, as it is laborious and empirical. Defined serum-free medium is essential for studies of growth factors and has major advantages in the commercial preparation of cell products and other applications.

More books from Springer Netherlands

Cover of the book The Urban Fabric of Crime and Fear by
Cover of the book Reforming Higher Education by
Cover of the book Liver Diseases in Infancy and Childhood by
Cover of the book Occult Atherosclerotic Disease by
Cover of the book Promoting Self-Change from Problem Substance Use by
Cover of the book Coral Reefs of the Gulf by
Cover of the book Biosensors for Direct Monitoring of Environmental Pollutants in Field by
Cover of the book The Logical Foundations of the Marxian Theory of Value by
Cover of the book Naturalistic Epistemology by
Cover of the book Laboratory animals in vaccine production and control by
Cover of the book Landscape Interfaces by
Cover of the book Approaches to Natural Language by
Cover of the book Personhood and Health Care by
Cover of the book Inborn Errors of Metabolism in Humans by
Cover of the book Computational Systems Neurobiology by
We use our own "cookies" and third party cookies to improve services and to see statistical information. By using this website, you agree to our Privacy Policy