High-Resolution Profiling of Protein-RNA Interactions

Nonfiction, Science & Nature, Technology, Engineering, Science, Biological Sciences, Health & Well Being, Medical
Cover of the book High-Resolution Profiling of Protein-RNA Interactions by Mathias Munschauer, Springer International Publishing
View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart
Author: Mathias Munschauer ISBN: 9783319162539
Publisher: Springer International Publishing Publication: March 14, 2015
Imprint: Springer Language: English
Author: Mathias Munschauer
ISBN: 9783319162539
Publisher: Springer International Publishing
Publication: March 14, 2015
Imprint: Springer
Language: English

The work reported in this book represents an excellent example of how creative experimentation and technology development, complemented by computational data analysis, can yield important insights that further our understanding of biological entities from a systems perspective. The book describes how the study of a single RNA-binding protein and its interaction sites led to the development of the novel ‘protein occupancy profiling’ technology that for the first time captured the mRNA sequence space contacted by the ensemble of expressed RNA binders. Application of protein occupancy profiling to eukaryotic cells revealed that extensive sequence stretches in 3’ UTRs can be contacted by RBPs and that evolutionary conservation as well as negative selection act on protein-RNA contact sites, suggesting functional importance. Comparative analysis of the RBP-bound sequence space has the potential to unravel putative cis-acting RNA elements without a priori knowledge of the bound regulators. Here, Dr. Munschauer provides a comprehensive introduction to the field of post-transcriptional gene regulation, examines state-of-the-art technologies, and combines the conclusions from several journal articles into a coherent and logical story from the frontiers of systems-biology inspired life science. This thesis, submitted to the Department of Biology, Chemistry and Pharmacy at Freie Universität Berlin, was selected as outstanding work by the Berlin Institute for Medical Systems Biology at the Max-Delbrueck Center for Molecular Medicine, Germany.

 

View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart

The work reported in this book represents an excellent example of how creative experimentation and technology development, complemented by computational data analysis, can yield important insights that further our understanding of biological entities from a systems perspective. The book describes how the study of a single RNA-binding protein and its interaction sites led to the development of the novel ‘protein occupancy profiling’ technology that for the first time captured the mRNA sequence space contacted by the ensemble of expressed RNA binders. Application of protein occupancy profiling to eukaryotic cells revealed that extensive sequence stretches in 3’ UTRs can be contacted by RBPs and that evolutionary conservation as well as negative selection act on protein-RNA contact sites, suggesting functional importance. Comparative analysis of the RBP-bound sequence space has the potential to unravel putative cis-acting RNA elements without a priori knowledge of the bound regulators. Here, Dr. Munschauer provides a comprehensive introduction to the field of post-transcriptional gene regulation, examines state-of-the-art technologies, and combines the conclusions from several journal articles into a coherent and logical story from the frontiers of systems-biology inspired life science. This thesis, submitted to the Department of Biology, Chemistry and Pharmacy at Freie Universität Berlin, was selected as outstanding work by the Berlin Institute for Medical Systems Biology at the Max-Delbrueck Center for Molecular Medicine, Germany.

 

More books from Springer International Publishing

Cover of the book Regulation of Membrane Na+-K+ ATPase by Mathias Munschauer
Cover of the book Osteomyelitis of the Foot and Ankle by Mathias Munschauer
Cover of the book Silicon Nanowire Transistors by Mathias Munschauer
Cover of the book Trade Marks and Free Trade by Mathias Munschauer
Cover of the book Extended Abstracts Summer 2016 by Mathias Munschauer
Cover of the book Nanoscience in Food and Agriculture 4 by Mathias Munschauer
Cover of the book The Monstrous-Feminine in Contemporary Japanese Popular Culture by Mathias Munschauer
Cover of the book Electrical Circuits in Biomedical Engineering by Mathias Munschauer
Cover of the book Design of FPGA-Based Computing Systems with OpenCL by Mathias Munschauer
Cover of the book Digital Milton by Mathias Munschauer
Cover of the book Queering Childhood in Early Modern English Drama and Culture by Mathias Munschauer
Cover of the book Hypogene Karst Regions and Caves of the World by Mathias Munschauer
Cover of the book Causal Analytics for Applied Risk Analysis by Mathias Munschauer
Cover of the book Extended Abstracts Fall 2012 by Mathias Munschauer
Cover of the book Principles of Mobile Communication by Mathias Munschauer
We use our own "cookies" and third party cookies to improve services and to see statistical information. By using this website, you agree to our Privacy Policy