Rapid Cell Magnetisation Using Cationised Magnetoferritin

Nonfiction, Science & Nature, Science, Biological Sciences, Biophysics, Cytology
Cover of the book Rapid Cell Magnetisation Using Cationised Magnetoferritin by Sara Correia Carreira, Springer International Publishing
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Author: Sara Correia Carreira ISBN: 9783319603339
Publisher: Springer International Publishing Publication: June 21, 2017
Imprint: Springer Language: English
Author: Sara Correia Carreira
ISBN: 9783319603339
Publisher: Springer International Publishing
Publication: June 21, 2017
Imprint: Springer
Language: English

Bringing together nanoscience with stem cell and bacterial cell biology, this thesis is truly interdisciplinary in scope. It shows that the creation of superparamagnetic nanoparticles inside a protein coat, followed by chemical functionalisation of the protein surface, provides a novel methodology for cell magnetisation using incubation times as short as one minute. Crucially, stem cell proliferation and multi-lineage differentiation capacity is not impaired after labelling. Due to the unspecific labelling mechanism, this thesis also shows that the same magnetic protein nanoparticles can be used for rapid bacterial magnetisation. Thus, it is possible to magnetically capture and concentrate pathogens from clinical samples quickly and highly efficiently.

 

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Bringing together nanoscience with stem cell and bacterial cell biology, this thesis is truly interdisciplinary in scope. It shows that the creation of superparamagnetic nanoparticles inside a protein coat, followed by chemical functionalisation of the protein surface, provides a novel methodology for cell magnetisation using incubation times as short as one minute. Crucially, stem cell proliferation and multi-lineage differentiation capacity is not impaired after labelling. Due to the unspecific labelling mechanism, this thesis also shows that the same magnetic protein nanoparticles can be used for rapid bacterial magnetisation. Thus, it is possible to magnetically capture and concentrate pathogens from clinical samples quickly and highly efficiently.

 

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