Laboratory Guide to Biochemistry, Enzymology, and Protein Physical Chemistry

A Study of Aspartate Transcarbamylase

Nonfiction, Science & Nature, Science, Chemistry, Physical & Theoretical, Biological Sciences, Biochemistry
Cover of the book Laboratory Guide to Biochemistry, Enzymology, and Protein Physical Chemistry by Raymond Chabaud, Marc le Maire, Guy Hervé, Springer US
View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart
Author: Raymond Chabaud, Marc le Maire, Guy Hervé ISBN: 9781461538202
Publisher: Springer US Publication: December 6, 2012
Imprint: Springer Language: English
Author: Raymond Chabaud, Marc le Maire, Guy Hervé
ISBN: 9781461538202
Publisher: Springer US
Publication: December 6, 2012
Imprint: Springer
Language: English

The study of a single well-chosen substance, here aspartate transcarb­ amylase, can provide an excellent basis for a laboratory course. The student is introduced to a variety of scientific ideas and to many experi­ mental and interpretive techniques. This enzyme is readily available, is relatively stable, has an extensive literature, and its behavior has many facets: substrate inhibition, a large change in structure upon homo­ tropic activation by substrates, allosteric stimulation by ATP, allosteric inhibition by CTP synergistic with VTP, positive cooperativity for sub­ strates, negative cooperativity for CTP binding, and dissociation and reassembly of subunits Cand R2 from the holoenzyme CI\5. In addition 3 6 to the known biochemical aspects of these properties, the results ob­ tained here can be interpreted in the light of the high-resolution X-ray diffraction structures of the T and R forms, the low-angle X-ray scattering results, and the large number of mutants now available by recombinant DNA methods. Future development of this course could also involve part of these methods, as well as the carefully chosen experiments described here. This approach resembles research more than the approaches one usually finds in biochemical laboratory courses. A consistent develop­ ment of ideas about a single enzyme, which shows so many facets in its behavior, is sure to hold the interest of the student. Moreover, one explores a depth, and reasons to move forward, that are an essential part of research.

View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart

The study of a single well-chosen substance, here aspartate transcarb­ amylase, can provide an excellent basis for a laboratory course. The student is introduced to a variety of scientific ideas and to many experi­ mental and interpretive techniques. This enzyme is readily available, is relatively stable, has an extensive literature, and its behavior has many facets: substrate inhibition, a large change in structure upon homo­ tropic activation by substrates, allosteric stimulation by ATP, allosteric inhibition by CTP synergistic with VTP, positive cooperativity for sub­ strates, negative cooperativity for CTP binding, and dissociation and reassembly of subunits Cand R2 from the holoenzyme CI\5. In addition 3 6 to the known biochemical aspects of these properties, the results ob­ tained here can be interpreted in the light of the high-resolution X-ray diffraction structures of the T and R forms, the low-angle X-ray scattering results, and the large number of mutants now available by recombinant DNA methods. Future development of this course could also involve part of these methods, as well as the carefully chosen experiments described here. This approach resembles research more than the approaches one usually finds in biochemical laboratory courses. A consistent develop­ ment of ideas about a single enzyme, which shows so many facets in its behavior, is sure to hold the interest of the student. Moreover, one explores a depth, and reasons to move forward, that are an essential part of research.

More books from Springer US

Cover of the book Paleobiogeography by Raymond Chabaud, Marc le Maire, Guy Hervé
Cover of the book Property Tax Reform in Developing Countries by Raymond Chabaud, Marc le Maire, Guy Hervé
Cover of the book Frontiers in Bioactive Lipids by Raymond Chabaud, Marc le Maire, Guy Hervé
Cover of the book AIDS and Women’s Reproductive Health by Raymond Chabaud, Marc le Maire, Guy Hervé
Cover of the book Pediatric Orthopedics by Raymond Chabaud, Marc le Maire, Guy Hervé
Cover of the book Viral Infections of Humans by Raymond Chabaud, Marc le Maire, Guy Hervé
Cover of the book The Emotional Brain by Raymond Chabaud, Marc le Maire, Guy Hervé
Cover of the book Reasoning in Boolean Networks by Raymond Chabaud, Marc le Maire, Guy Hervé
Cover of the book Mechanical Properties of Materials at Low Temperatures by Raymond Chabaud, Marc le Maire, Guy Hervé
Cover of the book Memory Design Techniques for Low Energy Embedded Systems by Raymond Chabaud, Marc le Maire, Guy Hervé
Cover of the book Hepatic Encephalopathy in Chronic Liver Failure by Raymond Chabaud, Marc le Maire, Guy Hervé
Cover of the book Handbook of Sample Preparation for Scanning Electron Microscopy and X-Ray Microanalysis by Raymond Chabaud, Marc le Maire, Guy Hervé
Cover of the book Hepatology and Transplant Hepatology by Raymond Chabaud, Marc le Maire, Guy Hervé
Cover of the book Finding Meaning in Dreams by Raymond Chabaud, Marc le Maire, Guy Hervé
Cover of the book Innovating for Healthy Urbanization by Raymond Chabaud, Marc le Maire, Guy Hervé
We use our own "cookies" and third party cookies to improve services and to see statistical information. By using this website, you agree to our Privacy Policy