How Free Cationic Polymer Chains Promote Gene Transfection

Nonfiction, Science & Nature, Technology, Textiles & Polymers, Science, Chemistry, Organic
Cover of the book How Free Cationic Polymer Chains Promote Gene Transfection by Yue Yanan, Springer International Publishing
View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart
Author: Yue Yanan ISBN: 9783319003368
Publisher: Springer International Publishing Publication: May 13, 2013
Imprint: Springer Language: English
Author: Yue Yanan
ISBN: 9783319003368
Publisher: Springer International Publishing
Publication: May 13, 2013
Imprint: Springer
Language: English

In this PhD thesis, Yue Yanan addresses a long-overlooked and critical question in the development of non-viral vectors for gene delivery. The author determines that those uncomplexed and cationic polymer chains free in the solution mixture of polymer and DNA facilitate and promote gene transfection. Furthermore, by using a combination of synthetic chemistry, polymer physics and molecular biology, Yue confirms that it is those cationic polymer chains free in the solution mixture, rather than those bound to DNA chains, that play a decisive role in intracellular trafficking. Instead of the previously proposed and widely accepted “proton sponge” model, the author's group propose a new hypothesis based on the results of several well-designed and decisive experiments. These results show that free polycationic chains with a length of more than ~10 nm are able to partially block the fusion between different endocytic vesicles, including the endocytic-vesicle-to-endolysosome pathway. This thesis is highly original and its results greatly deepen our understanding of polymer-mediated gene transfection. More importantly, it provides new insights into the rational design of next-generation superior polymeric gene-delivery vectors.

View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart

In this PhD thesis, Yue Yanan addresses a long-overlooked and critical question in the development of non-viral vectors for gene delivery. The author determines that those uncomplexed and cationic polymer chains free in the solution mixture of polymer and DNA facilitate and promote gene transfection. Furthermore, by using a combination of synthetic chemistry, polymer physics and molecular biology, Yue confirms that it is those cationic polymer chains free in the solution mixture, rather than those bound to DNA chains, that play a decisive role in intracellular trafficking. Instead of the previously proposed and widely accepted “proton sponge” model, the author's group propose a new hypothesis based on the results of several well-designed and decisive experiments. These results show that free polycationic chains with a length of more than ~10 nm are able to partially block the fusion between different endocytic vesicles, including the endocytic-vesicle-to-endolysosome pathway. This thesis is highly original and its results greatly deepen our understanding of polymer-mediated gene transfection. More importantly, it provides new insights into the rational design of next-generation superior polymeric gene-delivery vectors.

More books from Springer International Publishing

Cover of the book Nonlinearly Perturbed Semi-Markov Processes by Yue Yanan
Cover of the book The Roles of Accelerated Pavement Testing in Pavement Sustainability by Yue Yanan
Cover of the book Open Innovation Business Modeling by Yue Yanan
Cover of the book Bullying and Violence in South Korea by Yue Yanan
Cover of the book An Advanced Course in Computational Nuclear Physics by Yue Yanan
Cover of the book From Aardvarks to Zooxanthellae by Yue Yanan
Cover of the book Computability and Complexity by Yue Yanan
Cover of the book Physics of Semiconductor Devices by Yue Yanan
Cover of the book Discrete Fractional Calculus by Yue Yanan
Cover of the book Computations and Computing Devices in Mathematics Education Before the Advent of Electronic Calculators by Yue Yanan
Cover of the book Design of Interpretable Fuzzy Systems by Yue Yanan
Cover of the book The Hunt for Alien Life by Yue Yanan
Cover of the book The Future of FinTech by Yue Yanan
Cover of the book An Introduction to Design Science by Yue Yanan
Cover of the book Magnetic Nanostructures by Yue Yanan
We use our own "cookies" and third party cookies to improve services and to see statistical information. By using this website, you agree to our Privacy Policy