Elispot for Rookies (and Experts Too)

Nonfiction, Health & Well Being, Medical, Medical Science, Immunology, Science & Nature, Science, Other Sciences, Molecular Biology
Cover of the book Elispot for Rookies (and Experts Too) by Sylvia Janetzki, Springer International Publishing
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Author: Sylvia Janetzki ISBN: 9783319452951
Publisher: Springer International Publishing Publication: November 10, 2016
Imprint: Springer Language: English
Author: Sylvia Janetzki
ISBN: 9783319452951
Publisher: Springer International Publishing
Publication: November 10, 2016
Imprint: Springer
Language: English

This book provides basic, simple, and logical explanations for choices to be made to run the best Elispot possible. It allows the newcomer to truly understand the best options for specific protocol steps, reagents and materials, and provides even the experienced Elispot user with insight into best practices. The techniques presented here are supported by the author’s twenty-plus years of first-hand experience working with this assay, as well as the shared experiences of numerous colleagues and collaborators.

The enzyme-linked immunospot (Elispot) assay is a widely used technique to monitor cells on the single cell level for the release of analytes like cytokines, chemokines or immunoglobulins (antibodies), in response to particular stimuli. The most important feature of Elispot is its outstanding sensitivity, allowing the detection of specific cells in very low frequencies. The advantages of Elispot have resulted in it being widely adapted for use in research and translational applications in numerous fields including cancer, infectious diseases, autoimmunity, and transplantation.

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This book provides basic, simple, and logical explanations for choices to be made to run the best Elispot possible. It allows the newcomer to truly understand the best options for specific protocol steps, reagents and materials, and provides even the experienced Elispot user with insight into best practices. The techniques presented here are supported by the author’s twenty-plus years of first-hand experience working with this assay, as well as the shared experiences of numerous colleagues and collaborators.

The enzyme-linked immunospot (Elispot) assay is a widely used technique to monitor cells on the single cell level for the release of analytes like cytokines, chemokines or immunoglobulins (antibodies), in response to particular stimuli. The most important feature of Elispot is its outstanding sensitivity, allowing the detection of specific cells in very low frequencies. The advantages of Elispot have resulted in it being widely adapted for use in research and translational applications in numerous fields including cancer, infectious diseases, autoimmunity, and transplantation.

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