Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis

Nonfiction, Science & Nature, Science, Biological Sciences, Genetics, Technology, Engineering, Health & Well Being, Medical
Cover of the book Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis by Bin Zhuang, Springer Singapore
View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart
Author: Bin Zhuang ISBN: 9789811047534
Publisher: Springer Singapore Publication: July 20, 2017
Imprint: Springer Language: English
Author: Bin Zhuang
ISBN: 9789811047534
Publisher: Springer Singapore
Publication: July 20, 2017
Imprint: Springer
Language: English

This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an “in-situ” PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system’s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid “sample-in-answer-out” testing in routine clinical practice.

View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart

This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an “in-situ” PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system’s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid “sample-in-answer-out” testing in routine clinical practice.

More books from Springer Singapore

Cover of the book On Chinese Culture by Bin Zhuang
Cover of the book Bioreactors for Microbial Biomass and Energy Conversion by Bin Zhuang
Cover of the book Rural Labour Mobility in Times of Structural Transformation by Bin Zhuang
Cover of the book Being A Teacher in the 21st Century by Bin Zhuang
Cover of the book Advances in GAPDH Protein Analysis: A Functional and Biochemical Approach by Bin Zhuang
Cover of the book Neuromuscular Disorders by Bin Zhuang
Cover of the book Proceedings of the 1st AAGBS International Conference on Business Management 2014 (AiCoBM 2014) by Bin Zhuang
Cover of the book Ring Resonator Systems to Perform Optical Communication Enhancement Using Soliton by Bin Zhuang
Cover of the book ‘Out of School’ Ethnic Minority Young People in Hong Kong by Bin Zhuang
Cover of the book A Study of the Turning Point of China’s Debt by Bin Zhuang
Cover of the book Public Transport Passengers’ Behavioural Intentions by Bin Zhuang
Cover of the book Evolutionary Computing and Artificial Intelligence by Bin Zhuang
Cover of the book Smart Economy in Smart Cities by Bin Zhuang
Cover of the book Monte-Carlo Simulation-Based Statistical Modeling by Bin Zhuang
Cover of the book Content-Centric Networks by Bin Zhuang
We use our own "cookies" and third party cookies to improve services and to see statistical information. By using this website, you agree to our Privacy Policy