Cell Line Development

Nonfiction, Science & Nature, Science, Other Sciences, Molecular Biology, Biological Sciences, Genetics, Health & Well Being, Medical
Cover of the book Cell Line Development by , Springer Netherlands
View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart
Author: ISBN: 9789048122455
Publisher: Springer Netherlands Publication: August 11, 2009
Imprint: Springer Language: English
Author:
ISBN: 9789048122455
Publisher: Springer Netherlands
Publication: August 11, 2009
Imprint: Springer
Language: English

Mammalian cell lines command an effective monopoly for the production of therapeutic proteins that require post-translational modifications. This unique advantage outweighs the costs associated with mammalian cell culture, which are far grater in terms of development time and manufacturing when compared to microbial culture. The development of cell lines has undergone several advances over the years, essentially to meet the requirement to cut the time and costs associated with using such a complex hosts as production platforms.

This book provides a comprehensive guide to the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity, improve cell function, glycosylation and secretion and control apoptosis. It presents an overall picture of the current topics central to expression engineering including such topics as epigenetics and the use of technologies to overcome positional dependent inactivation, the use of promoter and enhancer sequences for expression of various transgenes, site directed engineering of defined chromosomal sites, and examination of the role of eukaryotic nucleus as the controller of expression of genes that are introduced for production of a desired product. It includes a review of selection methods for high producers and an application developed by a major biopharmaceutical industry to expedite the cell line development process. The potential of cell engineering approch to enhance cell lines through the manipulation of single genes that play important roles in key metabolic and regulatory pathways is also explored throughout.

View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart

Mammalian cell lines command an effective monopoly for the production of therapeutic proteins that require post-translational modifications. This unique advantage outweighs the costs associated with mammalian cell culture, which are far grater in terms of development time and manufacturing when compared to microbial culture. The development of cell lines has undergone several advances over the years, essentially to meet the requirement to cut the time and costs associated with using such a complex hosts as production platforms.

This book provides a comprehensive guide to the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity, improve cell function, glycosylation and secretion and control apoptosis. It presents an overall picture of the current topics central to expression engineering including such topics as epigenetics and the use of technologies to overcome positional dependent inactivation, the use of promoter and enhancer sequences for expression of various transgenes, site directed engineering of defined chromosomal sites, and examination of the role of eukaryotic nucleus as the controller of expression of genes that are introduced for production of a desired product. It includes a review of selection methods for high producers and an application developed by a major biopharmaceutical industry to expedite the cell line development process. The potential of cell engineering approch to enhance cell lines through the manipulation of single genes that play important roles in key metabolic and regulatory pathways is also explored throughout.

More books from Springer Netherlands

Cover of the book Tradition and Innovation by
Cover of the book Impact of Climate Change on Natural Resource Management by
Cover of the book Navigating Time and Space in Population Studies by
Cover of the book Perspectives on Teaching and Learning Chinese Literacy in China by
Cover of the book Progress in Anterior Eye Segment Research and Practice by
Cover of the book Mathematical Knowledge in Teaching by
Cover of the book Late Cenozoic Yushe Basin, Shanxi Province, China: Geology and Fossil Mammals by
Cover of the book The Philosophy of John Dewey by
Cover of the book I Domain Integrins by
Cover of the book Advances in Bifurcation and Degradation in Geomaterials by
Cover of the book An International Peace Court by
Cover of the book Quaternary of Scotland by
Cover of the book Work at the Boundaries of Science by
Cover of the book Hydrological Dimensioning and Operation of Reservoirs by
Cover of the book Innovative Approaches to the On-Site Assessment and Remediation of Contaminated Sites by
We use our own "cookies" and third party cookies to improve services and to see statistical information. By using this website, you agree to our Privacy Policy